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Primers used in this assay: C10T2 (sequence: 5ʹ-CCCCCCCCCCTT-3ʹ) to make cDNA, M13RC0T2 (sequence: 5ʹ-CAGGAAACAGC-TATGACCCCCCCCCCCTT-3ʹ) [45] to amplify the tail, forward primer (sequence: 5ʹ-GCGATGGCCCTGTCCTTTTA-3ʹ) to amplify both transcripts, reverse primer for the gene (sequence: 5ʹ-GGGACCAGTGTCTGGGTGATGT-3ʹ) to amplify only gene specific transcripts
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