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If confirmed severe gastroparesis is attributed to GLP-1 therapy, medication discontinuation is generally recommended, with most patients experiencing gradual symptom improvement over weeks to months
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Tissues were blocked in CAS block (Thermo Fisher Scientific) and incubated overnight with primary antibodies diluted in CAS block (Table 1): Primary antibodies for immunofluorescence Slides were washed 3 times in PBST, incubated overnight with secondary antibodies, and diluted in PBS/BSA 1% (Table 2): Secondary antibodies for immunofluorescence Slides were then washed once in PBS and closed with cover glass using ImmuGio mounting medium (DiaSorin, 2505-5)
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